human minlibcas9 library addgene (Addgene inc)
96
Structured Review
Addgene inc
human minlibcas9 library addgene
Human Minlibcas9 Library Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+minlibcas9+library+addgene/pSpCas9n(BB)-2A-GFP+(PX461)+(Plasmid+%2348140)/pm41564866-828-42-45
Average 96 stars, based on 269 article reviews
Human Minlibcas9 Library Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+minlibcas9+library+addgene/pSpCas9n(BB)-2A-GFP+(PX461)+(Plasmid+%2348140)/pm41564866-828-42-45
Average 96 stars, based on 269 article reviews
human minlibcas9 library addgene - by Bioz Stars,
2026-09
96/100 stars
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Recombinant:Article Title: CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistance. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cas9 CellSignalling Cat.# 14697; RRID: AB_2750916 CDX2 CellSignalling Cat.# 3977; RRID: AB_2077043 Vinculin CellSignalling Cat.# 13901; RRID: AB_2728768 Cleaved caspase 3 CellSignalling Cat.# 9661; RRID: AB_2341188 Caspase 8 CellSignalling Cat.# 9746; RRID: AB_2275120 Bacterial and virus strains Lucigen Endura TM ElectroCompetent Cells BioSearch Technologies Cat.# 60242-1 Stable Competent E. coli NEB Cat.#C3040I Biological samples CRC-9 tumor organoid and autologous PBMC cultures Cattaneo et al. 74 N/A Chemicals, peptides, and recombinant proteins IFN-γ Thermo Fisher Cat.# 300-02-100 μg IFN-β STEMCELL Technologies Cat.# 78113.1 TNF-α Thermo Fisher Cat.# PHC3011 IL-6 Thermo Fisher Cat.# PHC0064 Basement membrane extract (BME) R&D Systems Cat.# 3433-010-R1 Fixable viability dye - UV 455 eBioscience Cat.# Critical commercial assays CellTitre-Glo Promega Cat.#G7570 Foxp3/Transcription Factor Kit Thermo Fisher Cat.# 00-5521-00 Deposited data CRISPR-Cas9 screens SK-MEL-2 This paper ENA: ERP145139 CRISPR-Cas9 screens SK-MEL-2 This paper ENA: ERP145138 CRISPR-Cas9 screens HT-29 This paper ENA: ERP168780 CRISPR-Cas9 screens HT-29 This paper ENA: ERP142756 CRISPR-Cas9 screens HT-29 This paper ENA: ERP168941 CRISPR-Cas9 screens A375 This paper ENA: ERP140862 CRISPR-Cas9 screens A375 This paper ENA: ERP141157 RNA sequencing HT-29 This paper ENA: ERP148434 RNA sequencing VCaP This paper ENA: ERP146719 CRISPR-Cas9 screens CRC-9 This paper ENA: ERP171215 Experimental models: Cell lines HT-29 NCI RRID: CVCL_0320 .. VCaP ATCC RRID: CVCL_2235 B16-F10 ATCC RRID: CVCL_0159 Experimental models: Organisms/strains Mouse: C57BL/6 Charles River Laboratories N/A Oligonucleotides Primers are listed in Table S4 This paper N/A gRNAs are listed in Table S4 This paper N/A Recombinant DNA pSpCas9n(BB)-2A-GFP (PX461) Addgene #48140 Plasmid Preparation:Article Title: CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistance. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cas9 CellSignalling Cat.# 14697; RRID: AB_2750916 CDX2 CellSignalling Cat.# 3977; RRID: AB_2077043 Vinculin CellSignalling Cat.# 13901; RRID: AB_2728768 Cleaved caspase 3 CellSignalling Cat.# 9661; RRID: AB_2341188 Caspase 8 CellSignalling Cat.# 9746; RRID: AB_2275120 Bacterial and virus strains Lucigen Endura TM ElectroCompetent Cells BioSearch Technologies Cat.# 60242-1 Stable Competent E. coli NEB Cat.#C3040I Biological samples CRC-9 tumor organoid and autologous PBMC cultures Cattaneo et al. 74 N/A Chemicals, peptides, and recombinant proteins IFN-γ Thermo Fisher Cat.# 300-02-100 μg IFN-β STEMCELL Technologies Cat.# 78113.1 TNF-α Thermo Fisher Cat.# PHC3011 IL-6 Thermo Fisher Cat.# PHC0064 Basement membrane extract (BME) R&D Systems Cat.# 3433-010-R1 Fixable viability dye - UV 455 eBioscience Cat.# Critical commercial assays CellTitre-Glo Promega Cat.#G7570 Foxp3/Transcription Factor Kit Thermo Fisher Cat.# 00-5521-00 Deposited data CRISPR-Cas9 screens SK-MEL-2 This paper ENA: ERP145139 CRISPR-Cas9 screens SK-MEL-2 This paper ENA: ERP145138 CRISPR-Cas9 screens HT-29 This paper ENA: ERP168780 CRISPR-Cas9 screens HT-29 This paper ENA: ERP142756 CRISPR-Cas9 screens HT-29 This paper ENA: ERP168941 CRISPR-Cas9 screens A375 This paper ENA: ERP140862 CRISPR-Cas9 screens A375 This paper ENA: ERP141157 RNA sequencing HT-29 This paper ENA: ERP148434 RNA sequencing VCaP This paper ENA: ERP146719 CRISPR-Cas9 screens CRC-9 This paper ENA: ERP171215 Experimental models: Cell lines HT-29 NCI RRID: CVCL_0320 .. VCaP ATCC RRID: CVCL_2235 B16-F10 ATCC RRID: CVCL_0159 Experimental models: Organisms/strains Mouse: C57BL/6 Charles River Laboratories N/A Oligonucleotides Primers are listed in Table S4 This paper N/A gRNAs are listed in Table S4 This paper N/A Recombinant DNA pSpCas9n(BB)-2A-GFP (PX461) Addgene #48140 |